Does it matter if each reaction time is exceeded or less than the requested time?

Less reaction time will definitely affect the results. Without exceeding necessary time too much, the results should be fine. Eg. For 90 minutes reaction, do not react over 120 minutes; Do not exceed 10 minutes time for 5-minute reaction and 3 minutes time for 10-seconds reaction.

What does it mean by “room temperature?”  Would the test be affected with higher or lower temperature?

Temperature between 15~35℃ is so called room temperature. If room temperature is not within this range, inaccurate results may occur. Thus, it should be avoided.

What happen if I touched the detection stick by hand?

The bottom square detection window is the most untouchable space on the whole Detection Stick. Other space is fine to touch. However, if the square is touched, it is highly suggested to replace a new one.

Why cannot the door of Reaction Oven be left open for more than 10 seconds during the test?

If the door is left open for more than 10 seconds, heat would be lost and thus the temperature drop. This will affect the enzyme reaction during the test and cause inaccuracy. Thus, it should be avoided.

What does the “i-screen” reaction base on? What is the theory?

“i-screen” reaction is based on nucleic acid amplification system which amplifies and duplicates a large amount of viral nucleic acid followed by coloration to express the results. With this property, i-screen can tell you whether if the shrimp is infected with certain virus.

Would the largely amplified viral nucleic acid affect the healthy shrimp or the environment?

The largely amplified nucleic acid is only a small specific segment of the nucleic acid that applies to tests solely. It has no biology function or infection ability to any shrimp or environment.

What do I do with the reagent if there is a power failure occurred during the process?

• If no reagent is dissolved yet – the reagent can be refreezed. Please use these reagents prior to others.
• If the reagent is dissolved but no sample is taken yet – the reagent can be temporary restored back to the refrigerator (not in the freezer), and use it within 2 hours, or please discard it if it is not being used within 2 hours.
• If the reagent is dissolved, and sample is already taken - the reagent can be temporary restored back to the refrigerator (not in the freezer), and continue the test within 2 hours, or please discard it if test can’t be performed within 2 hours. The sample can be persevered at room temperature the longest for 6 hours. 
• If Tube 2 has already been reacted in the Reaction Oven – Gather all the unused reagents and Tube 2 inReacting Space and allow power to be resumed. If the power is recovered, please pre-heat the oven to 60℃for 30 minutes again. Place Tube 2 back to the oven for 90 minutes. If the power is not recovered within 2 hours, please discard this reagent set and start with a new kit.
• If Detection Stick has been applied– remaining heat in the oven would be sufficient to finish the rest steps.