Reacting Space

Move all the remaining test kits to Reacting Space. Place all Tube 2 on Tube Rack (Red) and move the rack into 60⁰C Reaction Oven which has been pre-heated for at least 30 minutes. Adjust timer to 90 minutes. Activate the Oven and start counting down.

Withdraw the rack after 90min. Prepare the Detection Stick and mark with sample number on every stick correspondingly. Insert Spotting Tip under the liquid surface of 2 and rest for 1 second. Later, touch the lower square shape detection window of Detection Stick for 1 second. Immediately discard the Spotting Tip to avoid cross contamination. Repeat the same step for another 2 tube with second Spotting Tip. Do not overlap two spotting areas. Screw the Detection Stick tightly onto Tube 3.

Put all Tube 3 on Tube Rack after shaking up-and-down for 5 times and place the Rack in 60℃ Reaction Oven for 5min. After 5 minutes, transfer and screw Detection Stick onto Tube 4. Submerge for 10s under room temperature.

Transfer and screw the Detection Stick onto Tube 5 and place all Tube 5 on Tube Rack. Place Put Tube Rack (Red) in 60℃Reaction Oven for 5 min. Later, transfer and screw Detection Stick onto Tube 6 and submerge for 10s in room temperature.

Transfer and screw the Detection Stick onto Tube 7 and place all Tube 7 on Tube rack. Put Tube Rack in 60⁰C Reaction Oven for 10 minutes. Retain Tube 6 with cap on. After 10 minutes, return the Detection Stick to Tube 6 and submerge for 10 seconds under room temperature. Take out Detection Stick and lay it horizontally on Tube Rack. Allow the detection window facing up and drying in Reaction Oven for 10 minutes. Lay Detection Stick horizontally on Tube rack. Allow the detection window facing up are drying in Reaction Oven for 10minutes. Withdraw Detection Stick for result interpretation.

Result Interpretation

Negative Result (No infection)：

It represents the whole test process is correct when there is a black line shows in Control Window is empty represents no virus infection.

Positive Resulte (Virus infection)︰

A black dot appears in Detection Window on Detection Stick.

Failed Result

There is no black line (Control window) nor black dot (Detection window) appears. Please refer to "Trouble Shooting" chapter if failure